metachat.pp.global_intensity_scaling

metachat.pp.global_intensity_scaling(adata_ref, adata_target, method='tic', scales=1e-05)

Perform global intensity scaling of adata_target to match adata_ref, using either total ion current (TIC) or root-mean-square (RMS) normalization.

Parameters

adata_ref

Reference dataset for scaling (e.g., negative ion mode).

adata_target

Target dataset to be scaled (e.g., positive ion mode).

method

Scaling method to use: - ‘tic’: scale by total ion current (sum of all intensities) - ‘rms’: scale by root-mean-square of intensities

scales

Optional global scaling factor applied to both datasets (default: 1e-5).

Returns

adata_refanndata.AnnData

Scaled reference dataset.

adata_targetanndata.AnnData

Scaled target dataset.